5U4L

RTA-V1C7_G29R-high-salt

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	64.970, 64.970, 215.968
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.898 - 2.500
R-factor	0.2116
Rwork	0.209
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2AAI and 4LGR
RMSD bond length	0.006
RMSD bond angle	0.981
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.110	0.033	2.793
Rmeas	0.116	0.035	2.949
Rpim	0.036	0.011	0.937
Total number of observations	197913
Number of reflections	19198
<I/σ(I)>	8.4
Completeness [%]	99.9	98.8	100
Redundancy	10.3	9.3	9.6
CC(1/2)		0.999	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	295	1.6 M ammonium dihydrogen phosphate, 20% glycerol, and 100 mM Tris pH 8.5