5U3Z
Human PPARdelta ligand-binding domain in complexed with specific agonist 10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.430, 94.750, 96.050 |
Unit cell angles | 90.00, 97.68, 90.00 |
Refinement procedure
Resolution | 42.530 - 1.720 |
R-factor | 0.1735 |
Rwork | 0.172 |
R-free | 0.21370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gwz |
RMSD bond length | 0.011 |
RMSD bond angle | 1.397 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9pre_1665) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.590 | 1.750 |
High resolution limit [Å] | 1.720 | 1.720 |
Number of reflections | 70808 | |
<I/σ(I)> | 9.8 | 0.8 |
Completeness [%] | 95.7 | 88.4 |
Redundancy | 5.8 | 2.7 |
CC(1/2) | 0.997 | 0.390 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277.15 | Bis-tris propane, potassium chloride, PEG 8000, 1,2-propandiol, EDTA, DTT |