5U2J
MORF double PHD finger (DPF) in complex with histone H3K14bu
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2016-06-21 |
| Detector | NOIR-1 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.540, 69.190, 69.930 |
| Unit cell angles | 90.00, 97.16, 90.00 |
Refinement procedure
| Resolution | 34.693 - 1.600 |
| R-factor | 0.1931 |
| Rwork | 0.191 |
| R-free | 0.23840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.904 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.700 |
| High resolution limit [Å] | 1.600 |
| Number of reflections | 40091 |
| <I/σ(I)> | 12.1 |
| Completeness [%] | 99.9 |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.1 M sodium citrate tribasic, 0.1 M sodium hepes |
