5U1S

Crystal structure of the Saccharomyces cerevisiae separase-securin complex at 3.0 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97919
Spacegroup name	P 32 2 1
Unit cell lengths	126.300, 126.300, 273.862
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.975 - 3.002
R-factor	0.1928
Rwork	0.190
R-free	0.25050
Structure solution method	SIRAS
RMSD bond length	0.003
RMSD bond angle	0.650
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.144	0.058
Rmeas	0.140	0.060
Rpim	0.050	0.017	0.723
Total number of observations	533070
Number of reflections	51156
<I/σ(I)>	6.3
Completeness [%]	99.8	99.2	99.6
Redundancy	10.4	12.3	7.6
CC(1/2)		0.999	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	0.25 M Na/K-phosphate (pH 6.0) and 14% (w/v) PEG 3350