5U1J

Structure of pNOB8 ParA bound to nonspecific DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-12
Detector	ADSC QUANTUM 210r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	68.300, 103.700, 104.000
Unit cell angles	90.00, 101.30, 90.00

Refinement procedure

Resolution	53.098 - 2.950
R-factor	0.2223
Rwork	0.220
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k5z
RMSD bond length	0.007
RMSD bond angle	0.944
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	103.660	3.110
High resolution limit [Å]	2.950	2.950
Rmerge		0.602
Rpim	0.050	0.416
<I/σ(I)>		1.9
Completeness [%]		96.8
Redundancy		2.9
CC(1/2)	0.995	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	298	20% PEG 4000, 10% isopropanol, 0.1 M Citrate pH 5.5