5U15
Crystal Structure of DH270.UCA3 (unliganded) from the DH270 Broadly Neutralizing N332-glycan Dependent Lineage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 98.533, 76.884, 137.097 |
| Unit cell angles | 90.00, 101.87, 90.00 |
Refinement procedure
| Resolution | 48.210 - 2.260 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5u0r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.084 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.214 | 2.300 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Rmerge | 0.096 | 0.878 |
| Number of reflections | 46692 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 20% PEG 4000, 100 mM Na acetate pH 5.0, 100 mM MgSO4 |
