5U0Q
RNA-DNA heptamer duplex with one 2'-5'-linkage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.554, 52.923, 27.622 |
| Unit cell angles | 90.00, 96.05, 90.00 |
Refinement procedure
| Resolution | 18.110 - 1.430 |
| R-factor | 0.15376 |
| Rwork | 0.153 |
| R-free | 0.17692 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5tko |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.755 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Rmerge | 0.055 | 0.614 |
| Number of reflections | 14036 | |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 10% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.040 M Sodium cacodylate trihydrate pH 5.5, 0.020 M Hexamine cobalt(III) chloride, 0.02 M Magnesium chloride hexahydrate |
