5U0B
Structure of full-length Zika virus NS5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2016-09-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 136.500, 197.000, 95.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 85.775 - 3.000 |
R-factor | 0.2327 |
Rwork | 0.231 |
R-free | 0.26800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k6m |
RMSD bond length | 0.002 |
RMSD bond angle | 0.511 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 85.775 | |
High resolution limit [Å] | 3.000 | |
Rmerge | 0.156 | 0.715 |
Rpim | 0.103 | 0.475 |
Number of reflections | 50661 | |
<I/σ(I)> | 6.4 | |
Completeness [%] | 97.6 | |
Redundancy | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 5.5 | 277 | 1.0 M ammonium sulfate plus 1% PE G 3350, 0.1 M Bis-Tris, pH 5.5 |