5TZM
59th Ig domain of human obscurin (OBSCN Ig59)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-04-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 60.980, 60.980, 47.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.490 - 1.177 |
R-factor | 0.165 |
Rwork | 0.164 |
R-free | 0.18520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2yz8 |
RMSD bond length | 0.033 |
RMSD bond angle | 1.459 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.490 | 1.219 |
High resolution limit [Å] | 1.177 | 1.177 |
Rmerge | 0.135 | 0.539 |
Number of reflections | 33764 | |
<I/σ(I)> | 22.98 | 5.07 |
Completeness [%] | 98.8 | 92.53 |
Redundancy | 19.5 | 10.7 |
CC(1/2) | 0.988 | 0.912 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 17% tacsimate, 0.1M HEPES pH 7.5, 4% PEG3350, 10 mg/mL C-terminal his-tagged Ig59 |