5TZ7
Crystal Structure of CurK Dehydratase D1169N Inactive Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.224, 94.512, 151.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.109 - 1.648 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 3kg9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.034 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.110 | 1.707 |
| High resolution limit [Å] | 1.648 | 1.648 |
| Number of reflections | 67404 | |
| <I/σ(I)> | 25.41 | 2.07 |
| Completeness [%] | 100.0 | 99 |
| Redundancy | 12.8 | 11.5 |
| CC(1/2) | 1.000 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.3 M Tri-Sodium Citrate, 30mm D(+) sucrose, 100mm tris ph 8.5 |
