5TYZ

DNA Polymerase Mu Product Complex, Mn2+ (960 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-11
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	59.934, 68.653, 109.999
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.982 - 1.977
R-factor	0.1774
Rwork	0.175
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.005
RMSD bond angle	0.680
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.010
High resolution limit [Å]	1.980	5.370	1.980
Rmerge	0.095	0.052	0.690
Total number of observations	272826
Number of reflections	32271
<I/σ(I)>	8.5
Completeness [%]	99.4	97.5	94
Redundancy	8.5	8.4	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000