5TYV

DNA Polymerase Mu Reactant Complex, Mn2+ (7.5 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-11
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	59.947, 68.752, 109.994
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.206 - 1.930
R-factor	0.1899
Rwork	0.189
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.003
RMSD bond angle	0.541
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.930	5.240	1.930
Rmerge	0.085	0.040	0.746
Total number of observations	336289
Number of reflections	34708
<I/σ(I)>	10.4
Completeness [%]	99.4	97.7	93.2
Redundancy	9.7	9.7	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000