5TYC

DNA Polymerase Mu Reactant Complex, 10mM Mg2+ (15 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-15
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	60.004, 68.577, 110.373
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.020 - 2.101
R-factor	0.1984
Rwork	0.196
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
RMSD bond length	0.003
RMSD bond angle	0.633
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.080
High resolution limit [Å]	2.040	5.540	2.040
Rmerge	0.110	0.039	0.583
Total number of observations	187227
Number of reflections	29583
<I/σ(I)>	6
Completeness [%]	99.7	97.3	98
Redundancy	6.3	6.3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000