5TY3
Crystal structure of K72A variant of Human Cytochrome c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.794, 37.902, 60.254 |
| Unit cell angles | 90.00, 116.79, 90.00 |
Refinement procedure
| Resolution | 23.830 - 1.250 |
| R-factor | 0.148 |
| Rwork | 0.147 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zcf |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.382 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.830 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.270 | |
| Number of reflections | 62478 | |
| <I/σ(I)> | 24.5 | 6.1 |
| Completeness [%] | 97.8 | 93.6 |
| Redundancy | 6.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 25% PEG 6000, 0.1 M TRIS PH 8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K |
