5TXV

HslU P21 cell with 4 hexamers

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	86.497, 420.860, 176.418
Unit cell angles	90.00, 98.56, 90.00

Refinement procedure

Resolution	49.187 - 7.086
R-factor	0.276
Rwork	0.274
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hqy
RMSD bond length	0.003
RMSD bond angle	0.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	7.220
High resolution limit [Å]	7.086	18.940	7.100
Rmerge	0.105	0.054	0.694
Number of reflections	18657
<I/σ(I)>	6.2
Completeness [%]	98.9	97.4	92.6
Redundancy	4.5	4	4.5
CC(1/2)		0.995	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM Bis-Tris pH 5.8 26% (W/V) PEG3350 260 mM Ammonium Sulfate