5TXL

STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING DATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	104
Detector technology	PIXEL
Collection date	2010-09-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	90.380, 133.846, 139.340
Unit cell angles	90.00, 97.30, 90.00

Refinement procedure

Resolution	48.075 - 2.501
R-factor	0.1937
Rwork	0.193
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v4i
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.067	0.530
Number of reflections	111859
<I/σ(I)>	15.6
Completeness [%]	98.7	88.1
Redundancy	4.1	2.7
CC(1/2)		0.619

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	PEG 8000, AMMONIUM SULFATE, MGCL2, GLYCEROL, SUCROSE