5TXL
STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING DATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 104 |
| Detector technology | PIXEL |
| Collection date | 2010-09-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.380, 133.846, 139.340 |
| Unit cell angles | 90.00, 97.30, 90.00 |
Refinement procedure
| Resolution | 48.075 - 2.501 |
| R-factor | 0.1937 |
| Rwork | 0.193 |
| R-free | 0.22450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3v4i |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.899 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.067 | 0.530 |
| Number of reflections | 111859 | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 98.7 | 88.1 |
| Redundancy | 4.1 | 2.7 |
| CC(1/2) | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | PEG 8000, AMMONIUM SULFATE, MGCL2, GLYCEROL, SUCROSE |
