5TXJ

Polymorphic form 1 of amyloid-beta derived peptide - IFAEDV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791
Spacegroup name	P 1
Unit cell lengths	9.518, 10.206, 20.064
Unit cell angles	96.22, 92.41, 85.32

Refinement procedure

Resolution	19.940 - 1.130
R-factor	0.146
Rwork	0.145
R-free	0.15700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6 RESIDUE BETA STRAND
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.100
Rmerge	0.068
Number of reflections	2605
<I/σ(I)>	14.7
Completeness [%]	92.9
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	reservoir contained 20% PEG 3350, 0.2M Ammonium Nitrate