5TXD
Structure of amyloid-beta derived peptide - NKGAIF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 29.402, 4.827, 26.612 |
Unit cell angles | 90.00, 102.04, 90.00 |
Refinement procedure
Resolution | 26.027 - 1.452 |
R-factor | 0.1758 |
Rwork | 0.174 |
R-free | 0.19120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6 residue alanine beta strand |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 26.030 |
High resolution limit [Å] | 1.450 |
Rmerge | 0.129 |
Number of reflections | 679 |
<I/σ(I)> | 6.8 |
Completeness [%] | 87.4 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | reservoir contained 20% PEG 3350, 0.2M Potassium Phosphate dibasic |