5TX2
Miniature TGF-beta2 3-mutant monomer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.459, 33.355, 54.131 |
| Unit cell angles | 90.00, 109.55, 90.00 |
Refinement procedure
| Resolution | 51.010 - 1.820 |
| R-factor | 0.2133 |
| Rwork | 0.209 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tgi |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.010 | 1.920 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.443 | |
| Number of reflections | 15033 | |
| <I/σ(I)> | 12.7 | 2.2 |
| Completeness [%] | 98.4 | 98.4 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 20% PEG 3350, 0.2 M sodium thiocyanate |
