5TWY

Structure of Maternal Embryonic Leucine Zipper Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	61.860, 80.020, 79.800
Unit cell angles	90.00, 109.95, 90.00

Refinement procedure

Resolution	58.148 - 2.910
R-factor	0.2885
Rwork	0.286
R-free	0.33020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.873
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.150	2.990
High resolution limit [Å]	2.910	2.910
Rmerge	0.041	0.614
Rmeas	0.049
Rpim	0.026
Total number of observations	53674
Number of reflections	15793
<I/σ(I)>	16.4
Completeness [%]	97.5	97
Redundancy	3.4	3.5
CC(1/2)	1.000	0.847

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 4000