5TWJ
Crystal Structure of RlmH in Complex with S-Adenosylmethionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-29 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.530, 37.360, 122.070 |
Unit cell angles | 90.00, 104.99, 90.00 |
Refinement procedure
Resolution | 29.479 - 2.299 |
R-factor | 0.1798 |
Rwork | 0.177 |
R-free | 0.22870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ns5 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.318 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.500 | 2.380 |
High resolution limit [Å] | 2.299 | 2.300 |
Rmerge | 0.077 | 0.755 |
Number of reflections | 26034 | 2583 |
<I/σ(I)> | 14.46 | 2.18 |
Completeness [%] | 98.6 | 98.47 |
Redundancy | 5.5 | 5.2 |
CC(1/2) | 0.999 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | 0.1 M Tris-bicine buffer (pH 8.5), 0.09 M NaF, 0.09 M NaBr, 0.09 M NaI, 20% v/v PEG 550 MME, 10% w/v PEG 20,000 in the presence of 2 mM S-Adenosylmethionine |