5TWH

human MOB1A bound to MST1 phosphorylated T367 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 43 21 2
Unit cell lengths	60.986, 60.986, 138.486
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.997 - 2.500
R-factor	0.2298
Rwork	0.228
R-free	0.26000
RMSD bond length	0.005
RMSD bond angle	0.795
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	56.000
High resolution limit [Å]	2.100
Number of reflections	16434
<I/σ(I)>	30
Completeness [%]	98.2
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	0.1 M MES pH 6.0, 0.2 M LiCl and 20% PEG 6000