5TWF

Regulation of protein interactions by MOB1 phosphorylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-05
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	C 2 2 21
Unit cell lengths	86.132, 86.160, 138.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.400 - 3.136
R-factor	0.2646
Rwork	0.263
R-free	0.29110
RMSD bond length	0.007
RMSD bond angle	1.269
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	3.100
Number of reflections	9254
<I/σ(I)>	13.5
Completeness [%]	99.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	298	0.1 M MES pH 5.5, 0.2 M NH4Cl, and 20% PEG 6000