5TVT

Structure of Maternal Embryonic Leucine Zipper Kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-12-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	56.690, 68.650, 105.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.362 - 2.280
R-factor	0.1917
Rwork	0.190
R-free	0.22940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.362
High resolution limit [Å]	2.280
Rmerge	0.094
Number of reflections	19214
<I/σ(I)>	12.54
Completeness [%]	99.6
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	24% PEG3350, 0.1 M MgCl, 0.1M HEPES pH 7.0