5TVL
Crystal structure of foldase protein PrsA from Streptococcus pneumoniae str. Canada MDR_19A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.315, 70.940, 116.626 |
| Unit cell angles | 90.00, 91.49, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.550 |
| R-factor | 0.22879 |
| Rwork | 0.225 |
| R-free | 0.29708 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.985 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.090 | 0.000 |
| Number of reflections | 43458 | |
| <I/σ(I)> | 20.04 | 0.9 |
| Completeness [%] | 98.7 | 90.9 |
| Redundancy | 9.8 | 6.2 |
| CC(1/2) | 1.000 | 0.580 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 11 | 293 | 2M Ammonium Sulfate, 0.05 M CAPSO pH 11 |
