5TVH
Crystal structure of AChBP from Aplysia californica complex with 2-aminopyrimidine at pH 8.0 spacegroup P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.780, 88.100, 115.500 |
Unit cell angles | 90.00, 97.50, 90.00 |
Refinement procedure
Resolution | 50.468 - 2.400 |
R-factor | 0.1928 |
Rwork | 0.191 |
R-free | 0.24720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2BYR.pdb |
RMSD bond length | 0.009 |
RMSD bond angle | 1.155 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 88.100 |
High resolution limit [Å] | 2.400 |
Rmerge | 0.457 |
Rpim | 0.178 |
Number of reflections | 50190 |
<I/σ(I)> | 16.2 |
Completeness [%] | 95.1 |
Redundancy | 7.4 |
CC(1/2) | 0.924 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 281 | 20% PEG 4000, 0.1M Tris pH 8.0, 0.2M NaCl |