5TV6
A. aeolicus BioW with pimelate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-31 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.486, 103.194, 113.805 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.829 - 2.456 |
R-factor | 0.1917 |
Rwork | 0.190 |
R-free | 0.22980 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.272 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | autoSHARP |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 30059 | |
<I/σ(I)> | 20.9 | |
Completeness [%] | 99.8 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 280 | 20-30% PEG4000 |