5TUW
Crystal structure of Orange Carotenoid Protein with partial loss of 3'OH Echinenone chromophore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.0781 |
| Spacegroup name | P 1 |
| Unit cell lengths | 82.947, 82.914, 87.438 |
| Unit cell angles | 89.79, 89.91, 60.04 |
Refinement procedure
| Resolution | 41.490 - 2.302 |
| R-factor | 0.1859 |
| Rwork | 0.185 |
| R-free | 0.23860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mg1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.012 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.102 | |
| Number of reflections | 85419 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 96.0 | 89 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 100 mM sodium acetate (pH 4.5) 16% PEG 8000 2% glycerol. |
