5TUL

Crystal structure of tetracycline destructase Tet(55)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-02-26
Detector	RDI CMOS_8M
Wavelength(s)	1.018211
Spacegroup name	P 21 21 2
Unit cell lengths	64.740, 124.410, 45.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.747 - 2.000
R-factor	0.1956
Rwork	0.193
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.797
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000	2.000
Number of reflections	25636
<I/σ(I)>	16.14
Completeness [%]	99.7
Redundancy	7.17
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Tris-HCl, pH 8.5, 25-27% PEG4000