5TUK
Crystal structure of tetracycline destructase Tet(51)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2015-05-21 |
Detector | RDI CMOS_8M |
Wavelength(s) | 1.000028 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 83.490, 81.690, 127.130 |
Unit cell angles | 90.00, 96.79, 90.00 |
Refinement procedure
Resolution | 19.904 - 1.850 |
R-factor | 0.1672 |
Rwork | 0.167 |
R-free | 0.19990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.224 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1616) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 144467 | |
<I/σ(I)> | 11.93 | |
Completeness [%] | 99.7 | |
Redundancy | 3.77 | |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES, pH 6.0, 10% PEG6000 |