5TUE

Crystal structure of tetracycline destructase Tet(50)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2015-07-23
Detector	RDI CMOS_8M
Wavelength(s)	1.000029
Spacegroup name	P 21 21 21
Unit cell lengths	50.940, 107.610, 152.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.825 - 2.100
R-factor	0.2293
Rwork	0.228
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.667
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1616)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100	2.100
Number of reflections	48896
<I/σ(I)>	15.07
Completeness [%]	98.1
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M MES, pH 6.0-6.5, 1.6-2.0 M ammonium sulfate, 2-10% 1,4-dioxane