5TUC
Crystal Structure of the Sus TBC1D15 GAP Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-26 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 139.000, 139.000, 175.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 87.620 - 2.500 |
R-factor | 0.2165 |
Rwork | 0.214 |
R-free | 0.25280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2qfz |
RMSD bond length | 0.004 |
RMSD bond angle | 0.873 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.610 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.172 | 1.254 |
Number of reflections | 35232 | |
<I/σ(I)> | 22.2 | 4.4 |
Completeness [%] | 100.0 | |
Redundancy | 39.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 50 mM MES sodium Salt (pH 6.5), 20% (w/v) PEG1000, 100 mM sodium chloride and 200 mM magnesium chloride |