5TUA
structure of a Na+-selective mutant of two-pore channel from Arabidopsis thaliana AtTPC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 88.535, 156.371, 217.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.589 - 3.300 |
| R-factor | 0.3109 |
| Rwork | 0.310 |
| R-free | 0.32290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e1j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.866 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 20921 |
| <I/σ(I)> | 17.7 |
| Completeness [%] | 90.3 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298.15 | 24-26% PEG400, 100-150 mM BaCl2, 100 mM HEPES pH7.0 or 100 mM MES pH6.0 |
