5TU8

Crystal structure of Staphylococcus epidermidis Aap G58-spacer-G513* (variant G5-spacer-variant G5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-28
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	43.623, 54.229, 184.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.250 - 2.330
R-factor	0.2117
Rwork	0.210
R-free	0.24590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fup
RMSD bond length	0.010
RMSD bond angle	1.230
Data reduction software	XDS (November 3, 2014)
Phasing software	PHASER (2.5.6)
Refinement software	BUSTER (2.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.250	2.413
High resolution limit [Å]	2.330	2.330
Rmerge	0.150	0.720
<I/σ(I)>		2.52
Completeness [%]		89
Redundancy		4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	100 mM sodium acetate pH 5 175 mM calcium chloride 22% PEG 6000