5TU7

Crystal structure of Staphylococcus epidermidis Aap G511-spacer-G513 (consensus G5-spacer-consensus G5)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03320
Spacegroup name	P 21 21 2
Unit cell lengths	43.200, 55.100, 185.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.812 - 3.340
R-factor	0.2596
Rwork	0.256
R-free	0.30670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.551
Data reduction software	XDS (May 1, 2016)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.812	3.460
High resolution limit [Å]	3.340	3.340
Rmerge	0.230	0.840
Number of reflections	6930
<I/σ(I)>	8.9	2.67
Completeness [%]	99.9	100
Redundancy	7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M sodium acetate pH 5.0 0.25 M calcium chloride 24% PEG 6000