5TU6
PagF prenyltransferase with cyclic[INPYLYP] and DMSPP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-08-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97856 |
Spacegroup name | P 65 |
Unit cell lengths | 116.490, 116.490, 43.580 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 2.220 |
R-factor | 0.18434 |
Rwork | 0.181 |
R-free | 0.23945 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.446 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.300 |
High resolution limit [Å] | 2.220 | 2.200 |
Number of reflections | 116928 | |
<I/σ(I)> | 12.7 | |
Completeness [%] | 93.5 | |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 280 | 20-25% PEG 3350 20 mM Tris-HCl pH=7.5 200 mM NaCl |