5TTY
PagF prenyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-10 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97872 |
Spacegroup name | P 65 |
Unit cell lengths | 116.930, 116.930, 43.642 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 1.800 |
R-factor | 0.19995 |
Rwork | 0.199 |
R-free | 0.22493 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.042 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.090 | 0.612 |
Number of reflections | 140616 | |
<I/σ(I)> | 14.1 | |
Completeness [%] | 99.8 | |
Redundancy | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 20-25% PEG 3350 20 mM Tris-Hcl pH 7.5 200 mM NaCl |