5TTX
Crystal structure of hydrogenase 2 maturation peptidase from Thaumarchaeota archaeon SCGC_AB-539-E09
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-29 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 131.966, 161.494, 101.702 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.979 - 2.401 |
R-factor | 0.2006 |
Rwork | 0.199 |
R-free | 0.22840 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.912 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.112 | 0.000 |
Number of reflections | 42616 | |
<I/σ(I)> | 20.4 | 1.84 |
Completeness [%] | 100.0 | 100 |
Redundancy | 5.7 | 5.7 |
CC(1/2) | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M sodium citrate, 20% PEG3350, cryo 15% glycerol |