5TTH
Heterodimeric SpyCatcher/SpyTag-fused zebrafish TRAP1 in ATP/ADP-hybrid state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 91.4 |
| Detector technology | CCD |
| Collection date | 2016-04-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.086, 95.711, 126.558 |
| Unit cell angles | 90.00, 134.59, 90.00 |
Refinement procedure
| Resolution | 49.000 - 3.200 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j0b |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 90.130 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 21244 |
| <I/σ(I)> | 9.1 |
| Completeness [%] | 83.0 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.19 M potassium acetate, 20-22% PEG3350, Benzamidine hydrochloride (5-25 mM) |
