5TTE

Crystal Structure of an RBR E3 ubiquitin ligase in complex with an E2-Ub thioester intermediate mimic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	108
Detector technology	PIXEL
Collection date	2015-11-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.282
Spacegroup name	P 65 2 2
Unit cell lengths	154.234, 154.234, 285.501
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.714 - 3.501
R-factor	0.2284
Rwork	0.226
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4kbc
RMSD bond length	0.005
RMSD bond angle	0.504
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.500
Rmerge	0.094
Number of reflections	24762
<I/σ(I)>	9.41
Completeness [%]	95.0
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.17	291	0.1M Sodium Cacodylate pH6.17, 0.35M Sodium Acetate, 10% Glycerol