5TSX

HIV-1 CA hexamer with NUP153 peptide - P1 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	57.333, 91.487, 150.047
Unit cell angles	89.54, 90.49, 96.08

Refinement procedure

Resolution	45.485 - 1.899
R-factor	0.1873
Rwork	0.185
R-free	0.22750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h47
RMSD bond length	0.007
RMSD bond angle	0.780
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	2.140	1.970
High resolution limit [Å]	1.900	2.050	1.900
Rmerge	0.072	0.381	0.753
Number of reflections	230655
<I/σ(I)>	9.6	2.1	1
Completeness [%]	96.5	95.7	92
Redundancy	1.7	1.7	1.6
CC(1/2)		0.692	0.316

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris, pH 9 12% PEG 8000