5TSV

HIV-1 CA hexamer with NUP153 peptide - R3 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-26
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	H 3
Unit cell lengths	151.924, 151.924, 69.646
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.824 - 2.502
R-factor	0.2082
Rwork	0.203
R-free	0.25120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h47
RMSD bond length	0.002
RMSD bond angle	0.475
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	3.050	2.540
High resolution limit [Å]	2.500	2.960	2.500
Rmerge	0.091	0.222	0.459
Number of reflections	15603
<I/σ(I)>	10.6	4.3	1.2
Completeness [%]	75.3	77.3	23.7
Redundancy	2.4	2.5	1.5
CC(1/2)		0.929	0.471

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Tris, pH 8.2 12% PEG 8000 2% Tacsimate