5TSO
CRYSTAL STRUCTURE OF GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM PIG MUSCLE COMPLEXED WITH ORTHOPHENANTHROLINE AT 1.90 ANGSTROM RESOLUTION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2005-04-15 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 1.00 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 86.480, 133.520, 210.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.540 - 1.900 |
R-factor | 0.133 |
Rwork | 0.132 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gd1 |
RMSD bond length | 0.019 |
RMSD bond angle | 2.019 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.540 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.066 | 0.159 |
Number of reflections | 87846 | |
<I/σ(I)> | 12 | |
Completeness [%] | 93.6 | 93.6 |
Redundancy | 4.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 273 | 0.005M 1,10-ORTHOPHENANTHROLINE, 0.05M HEPES, PH 8.0, 54.7%(W/V)AMMONIUM SULFATE,0.005 M EDTA, HANGING DROP, TEMPERATURE 277 K |