5TSN

Crystal structures of Norwalk virus polymerase bound to an RNA primer-template duplex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	81.760, 81.760, 189.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.908 - 2.100
R-factor	0.1765
Rwork	0.174
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bso
RMSD bond length	0.006
RMSD bond angle	0.863
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	61.830	2.210
High resolution limit [Å]	2.100	2.100
Rmerge	0.106	0.617
Number of reflections	38050
<I/σ(I)>	14.2	4.3
Completeness [%]	99.4	99.8
Redundancy	11.2	12
CC(1/2)	0.997	0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	14% PEG8000, 25% glycerol, 100 mM Tris-HCl, pH 7.0, 50 mM potassium chloride, 10 mM manganese chloride