5TSH
PilB from Geobacter metallireducens bound to AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-28 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97949322119491544 |
Spacegroup name | P 1 |
Unit cell lengths | 74.320, 99.960, 109.790 |
Unit cell angles | 112.99, 106.60, 88.91 |
Refinement procedure
Resolution | 45.755 - 2.300 |
R-factor | 0.1843 |
Rwork | 0.184 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1p9r |
RMSD bond length | 0.004 |
RMSD bond angle | 0.534 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.430 | |
Number of reflections | 253982 | |
<I/σ(I)> | 5.8 | 2 |
Completeness [%] | 96.0 | 95 |
Redundancy | 2.1 | 2.1 |
CC(1/2) | 0.995 | 0.639 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350, magnesium formate, Tris pH 7.0 |