5TSE

2.35 Angstrom Crystal Structure Minor Lipoprotein from Acinetobacter baumannii.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 41
Unit cell lengths	68.806, 68.806, 96.586
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.320 - 2.350
R-factor	0.2182
Rwork	0.216
R-free	0.26280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bf2
RMSD bond length	0.009
RMSD bond angle	1.463
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.058	0.723
Number of reflections	18791
<I/σ(I)>	31.5	2.1
Completeness [%]	100.0	99.8
Redundancy	7.5	5.5
CC(1/2)		0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	295	Protein: 15.9 mg/ml, 0.01M Tris HCl (pH 8.3), Screen: JCSG+ (A8), 0.2M Ammonium formate, 20% (w/v) PEG 3350.