5TS5
Crystal structure of L-amino acid oxidase from Bothrops atrox
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-28 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.4586 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.640, 123.920, 105.080 |
Unit cell angles | 90.00, 96.03, 90.00 |
Refinement procedure
Resolution | 34.572 - 2.300 |
R-factor | 0.1826 |
Rwork | 0.180 |
R-free | 0.23030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1reo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.629 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.840 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.146 | 0.557 |
Number of reflections | 80024 | |
<I/σ(I)> | 6.4 | 1.7 |
Completeness [%] | 96.5 | 93.4 |
Redundancy | 2.1 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5 | 295 | zinc sulfate heptahydrate, sodium cacodylate pH 5, polyethylene glycol monomethyl ether 550 |