5TRP
Crystal Structure of the Unliganded DH270 Cooperating Lineage Member DH272
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-23 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 60.862, 124.502, 146.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.389 - 2.692 |
| R-factor | 0.2139 |
| Rwork | 0.211 |
| R-free | 0.25610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5f6i 4lss |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.802 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.7) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.389 | 50.000 | 2.730 |
| High resolution limit [Å] | 2.680 | 7.270 | 2.680 |
| Rmerge | 0.119 | 0.051 | 0.638 |
| Rmeas | 0.164 | 0.070 | 0.879 |
| Rpim | 0.113 | 0.048 | 0.601 |
| Total number of observations | 47496 | ||
| Number of reflections | 15432 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 96.0 | 92.3 | 96.1 |
| Redundancy | 1.7 | 1.8 | 1.6 |
| CC(1/2) | 0.989 | 0.538 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 2.5 M AmSO4, 100 mM Na Acetate, pH 5.0 |
