5TRG
Structure of Mycobacterium tuberculosis proteasome in complex with N,C-capped dipeptide DPLG-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.045, 219.087, 137.894 |
| Unit cell angles | 90.00, 104.87, 90.00 |
Refinement procedure
| Resolution | 49.530 - 2.804 |
| R-factor | 0.1632 |
| Rwork | 0.160 |
| R-free | 0.21560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hfa |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.292 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.077 | 0.490 |
| Number of reflections | 167090 | |
| <I/σ(I)> | 8.1 | 2.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.988 | 0.776 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 60 mM sodium citrate (pH 6.2), 14% PEG-3350, |
