5TRC
Crystal structure of phosphorylated AC3-AC5 domains of yeast acetyl-CoA carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.433, 93.191, 110.944 |
| Unit cell angles | 90.00, 99.57, 90.00 |
Refinement procedure
| Resolution | 46.569 - 2.900 |
| R-factor | 0.2262 |
| Rwork | 0.223 |
| R-free | 0.28660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cs4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.313 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 6.240 | 2.900 |
| Rmerge | 0.051 | 0.029 | 0.504 |
| Rmeas | 0.063 | ||
| Rpim | 0.037 | ||
| Total number of observations | 68733 | ||
| Number of reflections | 25115 | ||
| <I/σ(I)> | 13.7 | ||
| Completeness [%] | 98.3 | 91.4 | 99.2 |
| Redundancy | 2.7 | 2.7 | 2.7 |
| CC(1/2) | 0.997 | 0.798 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES (pH 7.5), 3% (v/v) MPD, 2.5 mM sodium citrate, and 5% (v/v) glycerol |
